3 edition of heterogenous testis transplant problem as applied to white mice and rats. found in the catalog.
heterogenous testis transplant problem as applied to white mice and rats.
George Russell Crisler
Written in English
|LC Classifications||QP89 .C74|
|The Physical Object|
|Number of Pages||11|
|LC Control Number||81459907|
SCIENTISTS ARE preparing to carry out the first testicle transplant on a boy undergoing cancer treatment who would otherwise have become the operation is successful medical. Transplantation of testicular cells and tissues has been studied for the investigation of immunology of the testis, which is an immunologically privileged organ. However, reports of transplant of the testis at organ level have been extremely limited because of technical difficulties of the orthotopic testis transplantation (OTT) in experimental animals.
The applied doses were selected according to. Animals. This study was performed on 30 young male Wistar rats, weighing about 75–85 g Animals were obtained from the animal house of the King Fahad Center for Medical Research, King Abdulaziz University in Jeddah. The testis-to-neck transplant was performed on 12 rats with a success rate of percent. The OTT success rate, on the other hand, was lower at 71 percent, with a total operative time of more.
The use of transgenic rats vs mice to facilitate genetic research has been an important choice that researchers have needed to consider since the first transgenic rats were developed. Before that, laboratory mice and their use in embryonic stem cell studies dominated the world of genetics. Abstract This study explores the potential effects of interspecific differences in breeding systems on testis organisation and sperm morphology of native murid rodents. It poses the question – what.
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CRISLER, G. The heterogenous testis transplant problem as applied t o white rats and mice. DERNBY, K. A study of autolysis of animal tissues. Introduction. The testis is an immunologically unique site because new differentiation autoantigens-bearing spermatids and spermatozoa that appear in the seminiferous tubules long after the establishment of immune tolerance survive with no rejection by self-immunity under healthy conditions [1, 2].It became evident that transplantation of testicular tissue fragments from newborn donors in Cited by: 2.
Heterogeneous testicular echotexture at ultrasound may be the result of a variety of underlying pathology: seminiferous tubular atrophy - can occur in around 14% of middle aged to elderly patients 2 testicular trauma orchitis.
The Heterogeneous testis transplant problem as applied to white mice and rats. Techniques for homologous and xenogeneic transplantation of testicular stem cells have been described for mice. Retransplantation was performed by microinjection into the seminiferous tubules.
This technique succeeded in filling up to 80% of the surface area of the mouse testis (Brinster and Avarbock, ).Cited by: A veteran of the US Armed Forces has a new penis and scrotum after the most extensive penis transplant yet, Johns Hopkins Hospital announced this week.
Not included in the transplant. Testicles. Marked testis cells from transgenic rats were transplanted to the testes of immunodeficient mice, and in all of 10 recipient mice (in 19 of 20 testes), rat spermatogenesis occurred.
A ratT-cell leukemia model was used to study the safety of germ cell transplantation as a mean of preventing infertility in males undergoing gonadotoxic cancer treatment.
Donor germ cells were harvested from the testes of terminally ill leukemic rats and were either used directly or cryopreserved and thawed before transplantation by rete testis microinjection. In this study, we used marmoset as a pre-clinical animal model. ITT was obtained from two 6-month-old co-twin marmosets.
A total of testis tissue pieces (~– mm 3 each) were transplanted into the testicular parenchyma (intratesticular; n = 40) or under the dorsal skin (ectopic; n = ) of 4-week-old immunodeficient Swiss Nu/Nu mice (n = 20).
Testicles were obtained at autopsy from a series of 25 male cadavers (age range, years; mean, 62 years). Eight subjects had a history of cancer. Ex vivo sonography was performed and two board-certified radiologists graded the testis by consensus as normal, heterogeneous, or “other abnormality” (cyst, dilated rete, echogenic focus, or.
Generally, the rete testis is very small in rodents and not well visible in the histological section. In mice it is slightly more prominent than in rats and hyperplasia of the rete testis can be observed in older animals.
For inclusion of the rete testis in the histological section orientation is given by the vasculature. Both fresh and cryopreserved testis cells were used for transplantation, and all donor rats were heterozygous for the transgene.
In case of cryopreservation, cells were stored in liquid nitrogen for 29 to 30 days. After thawing, ≈70–80% ( ± %, mean ± SEM, n = 5) of the cryopreserved cells were viable, as assessed by trypan blue.
More information: Kai Yi et al. Development of heterotopic transplantation of the testis with the epididymis to evaluate an aspect of testicular immunology in rats. Start studying VTT Mice and Rat Test.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. Animals. Adult nude (Swiss nu‐nu/Ncr) mice bred at the University of Texas M. Anderson Cancer Center (Houston, Tex) were used as were transgenic mice (Tg (ACTB‐EGFP)1Osb/J) on a C57BL/6 background that express green fluorescent protein (GFP) under the control of a chicken β‐actin promoter (Okabe et al, ) (Jackson Laboratories; Bar Harbor, Me) or transgenic rats.
An undescended testicle is one that has failed to complete this trip. In some cases, the testicle stays high in the abdomen, and autotransplantation is the surgical procedure to correct this. An untreated undescended testicle can lead to increased chances of infertility or.
More than million mice and rats are killed in U.S. laboratories every year. They are abused in everything from toxicology tests (in which they are slowly poisoned to death) to painful burn experiments to psychological experiments that induce terror, anxiety, depression, and helplessness.
Evolution of the testis and spermatozoon in mice and rats (Subfamily Murinae) in the absence of sperm competition E. Peirce. Adelaide Medical School, Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, SA, Australia.
Search for more papers by this author. A British man has become the first in the world to successfully father a child after a testicular transplant following life-saving chemotherapy. The man suffered from Hodgkin’s lymphoma – a. The bodyweights of mice were remarkably altered at 10 and μg/L MC-LR compared with the control after exposed for days.
A significant decrease was firstly observed after and days of treatment with and 10 μg/L MC-LR respectively (Fig. 1A).Two testes of one mice were weighted and the organ coefficients of testis was the ratio of testes to body weight.
Lab rats probably don’t have many deep thoughts. But one of these days, right as a scientist is transplanting a testicle onto a rats’s neck, one of those little furry guys is going to ask.testes of terminally ill leukemic rats and were either used directly or cryopreserved and thawed before transplantation by rete testis microin-jection.
All rats transplanted with testicular cells from leukemic donors developed signs of terminal rat T-cell leukemia, whereas control animals remained healthy.tex and medulla, seminiferous tubules and rete testis) is important to ensure an adequate histologic evaluation of all potential target sites in a given organ.
All these requirements were set in the frame of cost effectiveness, i.e. to gain a maximum of information with an acceptable investment of .